Assay Kit
⭐ Best Seller
New
OrganelleAge™ Gene Validation Suite
Catalog #: TOVP-001
Integrated Translational Validation Package for OrganelleAgeClock and Disease-Reversal Modeling.
Kit Components & Pricing
| Item | Description | Cost (USD) |
|---|---|---|
| 1 | Silencer Select siRNA / CRISPR perturbation reagents | $1,050 |
| 2 | TaqMan qPCR assays and master mix | $820 |
| 3 | MitoSOX mitochondrial ROS assay reagents | $540 |
| 4 | MitoTracker mitochondrial morphology dyes | $460 |
| 5 | Image-iT Cell Painting Kit | $1,320 |
| 6 | CellROX oxidative stress reagents | $420 |
| 7 | Basic confocal consumables and staining reagents | $626 |
| Total | $5,236 | |
* Prices are approximate research-use estimates. Confocal microscopy instrumentation assumed available.
Standard Validation Workflow
- 1Select target gene predicted by OrganelleAgeClock
- 2Perform siRNA or CRISPR perturbation
- 3Confirm knockdown using TaqMan qPCR
- 4Apply disease stress model (high glucose, TNF-α, palmitate, Ang II, hypoxia, etc.)
- 5Perform confocal imaging with MitoSOX, MitoTracker, and Cell Painting reagents
- 6Quantify mitochondrial ROS, morphology, fragmentation, and cell-state remodeling
- 7Compare treated cells against disease baseline and healthy control
- 8Calculate predicted rejuvenation or acceleration effect in biological years
Key Readouts
- qPCR knockdown efficiency ≥70%
- Reduction in mitochondrial ROS
- Improved mitochondrial morphology
- Reduced inflammatory or senescence phenotype
- Cell Painting profile shifts toward healthy-state embedding
- Reproducibility across biological replicates
Recommended Cell Models
- Primary human endothelial cells
- Primary human fibroblasts
- Human macrophages
- hiPSC-derived cardiomyocytes
- Adipocytes for organ-talk studies